Anastasia Ryzhkova

• Knows the methods of the embryological stage of the cycle ART.
• Collection and denudation of oocyte-cumulus complexes.
• Fertilization (IVI, ICSI, IMSI, PICSI).
• Cultivation and embryo quality assessment;
• Vitrification of oocytes and embryos.
• Defrosting of oocytes and embryos.
• Embryo transfer;
• Auxiliary hatch;
• Trophectoderm biopsy.
• Visualization division spindles;
• Activation of eggs by Ca2+ ionophore;
• Preliminary assessment of sperm quality, macro- and microscopic analysis.
• Obtaining spermatozoa from a testicular biopsy (TESA, TESE).
• Microfluidics is the processing of spermatozoa on microfluidic chips.
• Preparation of the ejaculate for ART programs.

• 2012 — graduated from the Biological Faculty of the K.I. Scriabin Moscow State Medical University with a degree in Biochemistry, with honors. The WRC was completed on the topic: "The effect of Arf6 small GTPase on the characteristics of HT1080 fibrosarcoma cells".
• 2014 — received a Master's degree in Biology, cum laude. A research paper on the topic: "Association of mitochondrial genome mutations with asymptomatic atherosclerosis in women."

Postgraduate education

• 2018 — completed postgraduate studies at the K.I. Scriabin MGAVMiB-MBA, qualified as a Researcher. The teacher is a researcher. NCR "Cellular mechanisms of connection of mitochondrial mutations with atherosclerosis"

• THE STUDY OF MUTATIONS OF MITOCHONDRIAL RNA GENES IN LIPOFIBROUS PLAQUES OF THE HUMAN AORTIC INTIMA Sazonova M.A., Sinev V.V., Ryzhkova A.I., Sazonova M.D. In the collection: PROMISING SCIENTIFIC RESEARCH: EXPERIENCE, PROBLEMS AND DEVELOPMENT PROSPECTS. Collection of scientific articles based on the materials of the VI International Scientific and Practical Conference. Ufa, 2021. pp. 19-27.
• A NEW MARKER FOR THE STUDY OF ATHEROSCLEROTIC LESIONS: THE LEVEL OF HETEROPLASMY OF FOUR MITOCHONDRIAL MUTATIONS OF NADH DEHYDROGENASE SUBUNITS Sazonova M.A., Sinev V.V., Ryzhkova A.I., Sazonova M.D. In the collection: MODERN SCIENTIFIC RESEARCH: THEORY, METHODOLOGY, PRACTICE. Collection of scientific articles based on the materials of the VI International Scientific and Practical Conference. Ufa, 2021. pp. 29-35.
• CREATION OF HYBRID CULTURES CONTAINING A MUTATION OF THE M.1555A gt;G MITOCHONDRIAL GENOME (MT-RNR1 GENE), WHICH HAS A PROTECTIVE EFFECT IN ATHEROSCLEROSIS Sazonova M.A., Sinev V.V., Ryzhkova A.I., Sazonova M.D., Doroshchuk N.A., Kirichenko T.V., Orekhov A.N., Sobenin I.A. Pathological physiology and experimental therapy. 2021. Vol. 65. No. 4. pp. 121-127.
• A NEW MARKER OF HUMAN AGING: THE LEVEL OF HETEROPLASMIC MUTATIONS OF THE MITOCHONDRIAL GENOME Sinev V.V., Ryzhkova A.I., Sazonova M.D., Doroshchuk N.A., Kirichenko T.V., Karagodin V.P., Orekhov A.N., Sobenin I.A., Sazonova M.A.Pathological physiology and experimental therapy. 2021. Vol. 65. No. 2. pp. 4-9.
• MUTATIONS OF MTDNA IN SOME VASCULAR AND METABOLIC DISEASES Sazonova M.A., Ryzhkova A.I., Sinyov V.V., Sazonova M.D., Doroschuk N.A., Karagodin V.P., Orekhov A.N., Sobenin I.A., Kirichenko T.V.
• Current Pharmaceutical Design. 2021. Vol. 27. No. 2. pp. 177-184.
• SOME MOLECULAR AND CELLULAR STRESS MECHANISMS ASSOCIATED WITH NEURODEGENERATIVE DISEASES AND ATHEROSCLEROSIS Sazonova M.A., Sinyov V.V., Ryzhkova A.I., Sazonova M.D., Kirichenko T.V., Khotina V.A., Karagodin V.P., Orekhov A.N., Sobenin I.A., Khasanova Z.B., Doroschuk N.A. International Journal of Molecular Sciences. 2021. Vol. 22. No. 2. pp. 1-26.
• AN ORIGINAL BIOMARKER FOR THE RISK OF DEVELOPING CARDIOVASCULAR DISEASES AND THEIR COMPLICATIONS: TELOMERE LENGTH Doroschuk N.A., Doroschuk A.D., Ryzhkova A.I., Sinyov V.V., Sazonova M.D., Khotina V.A., Orekhov A.N., Sobenin I.A., Sazonova M.A., Postnov A.Y. Toxicology Reports. 2021. Vol. 8. pp. 499-504.
• THE CONNECTION OF AUTOPHAGY WITH THE PATHOGENESIS OF VARIOUS DISEASES Sazonova M.A., Sinev V.V., Ryzhkova A.I., Sazonova M.D. In THE collection: INNOVATIVE POTENTIAL OF SCIENCE DEVELOPMENT IN THE MODERN WORLD. Collection of works based on the materials of the III All-Russian competition of scientific research papers. Ufa, 2020. pp. 12-20.
• THE MAIN MECHANISMS OF MACROAUTOPHAGY Sazonova M.A., Sinev V.V., Ryzhkova A.I., Sazonova M.D. In the collection: FUNDAMENTAL AND APPLIED APPROACHES TO SOLVING SCIENTIFIC PROBLEMS. Collection of scientific articles based on the materials of the IV International Scientific and Practical Conference. Ufa, 2020. pp. 19-26.
• AUTOPHAGY: A MODERN CLASSIFICATION Sazonova M.A., Sinev V.V., Ryzhkova A.I., Sazonova M.D. In the collection: INNOVATIONS IN SCIENCE AND PRACTICE. Collection of scientific articles based on the materials of the IV International Scientific and Practical Conference. Ufa, 2020. pp. 19-27.
• CREATION OF HYBRID CELL CULTURES CONTAINING A SINGLE NUCLEOTIDE SUBSTITUTION IN THE MT-TL2 GENE ASSOCIATED WITH ATHEROSCLEROSIS Sazonova M.A., Sinev V.V., Ryzhkova A.I., Sazonova M.D., Doroshchuk N.A., Kirichenko T.V., Karagodin V.P., Orekhov A.N., Sobenin I.A. Pathological physiology and experimental therapy. 2020. Vol. 64. No. 4. pp. 140-147.
• IMPACT OF MITOCHONDRIAL DNA MUTATIONS ON CAROTID INTIMA-MEDIA THICKNESS IN THE NOVOSIBIRSK REGION Kirichenko T.V., Orekhov A.N., Sobenin I.A., Ryzhkova A.I., Sinyov V.V., Sazonova M.D., Orekhova V.A., Karagodin V.P., Sazonova M.A., Gerasimova E.V., Voevoda M.I., Ragino Y.I. Life. 2020. Vol. 10. No. 9. pp. 1-9.
• HETEROPLASMY LEVEL ANALYSIS OF MTDNA MUTATIONS IN INTIMA-MEDIAL LAYER OF NOVOSIBIRSK REGION PATIENTS Ryzhkova A.I., Sinyov V.V., Sazonova M.D., Khasanova Z.B., Doroschuk N.A., Nikitina N.A., Sobenin I.A., Orekhov A.N., Sazonova M.A. Atherosclerosis. 2019. Vol. 287. p. e163.
• VARIABILITY OF HETEROPLASMY OF SOME MITOCHONDRIAL GENOME MUTATIONS IN DIFFERENT HUMAN TISSUES Sinyov V.V., Ryzhkova A.I., Sazonova M.D., Khasanova Z.B., Doroschuk N.A., Nikitina N.A., Sobenin I.A.,Orekhov A.N., Sazonova M.A. Atherosclerosis. 2019. Vol. 287. p. e164.
• ANALYSIS OF MTDNA MUTATIONS IN ATHEROSCLEROTIC PLAQUES IN INDIVIDUALS FROM NOVOSIBIRSK Sazonova M.D., Ryzhkova A.I., Sinyov V.V., Khasanova Z.B., Doroschuk N.A., Nikitina N.A., Sobenin I.A., Orekhov A.N., Sazonova M.A. Atherosclerosis. 2019. Vol. 287. p. e166.
• CREATION OF CULTURES CONTAINING MUTATIONS LINKED WITH CARDIOVASCULAR DISEASES USING TRANSFECTION AND GENOME EDITING Sazonova M.A., Sinyov V.V., Khasanova Z.B., Nikitina N.A., Sobenin I.A., Ryzhkova A.I., Sazonova M.D., Orekhov A.N., Karagodin V.P. Current Pharmaceutical Design. 2019. Vol. 25. No. 6. pp. 693-699.
• CREATION OF CYBRID CULTURES CONTAINING MTDNA MUTATIONS M.12315G>A AND M.1555G>A, ASSOCIATED WITH ATHEROSCLEROSIS Sazonova M.A., Ryzhkova A.I., Sazonova M.D., Karagodin V.P., Orekhov A.N., Sobenin I.A., Sinyov V.V., Khasanova Z.B., Shkurat T.P. Biomolecules. 2019. Vol. 9. No. 9. p. 499.
• MTDNA MUTATIONS LINKED WITH LEFT VENTRICULAR HYPERTROPHY Sazonova M.A., Sinyov V.V., Khasanova Z.B., Sobenin I.A., Ryzhkova A.I., Sazonova M.D. Vessel Plus. 2019. Vol. 3.
• MITOCHONDRIAL MUTATIONS ASSOCIATED WITH CARDIAC ANGINA Sazonova M.A., Ryzhkova A.I., Sazonova M.D., Sobenin I.A., Orekhov A.N., Sinyov V.V., Nikitina N.N., Shkurat T.P. Vessel Plus. 2019. Vol. 3.
• THE ROLE OF POINT MUTATIONS OF THE MITOCHONDRIAL GENOME IN THE DEVELOPMENT OF MITOCHONDRIAL CYTOPATHIES Ryzhkova A.I., Sazonova M.A., Sinev V.V., Khasanova Z.B., Postnov A.Yu., Orekhov A.N., Sobenin I.A. In the book: Arterial hypertension 2018 at the crossroads of opinions. Abstracts of the XIV All-Russian Congress. 2018. pp. 74-75.
• DETECTION OF HOMOPLASMIC MTDNA MUTATIONS BY NGS METHOD IN PATIENTS WITH CAROTID ATHEROSCLEROSIS Sazonova M.A., Sinyov V.V., Ryzhkova A.I., Sazonova M.D., Zhelankin A.V., Khasanova Z.B., Postnov A.Y., Orekhov A.N., Sobenin I.A. Atherosclerosis. 2018. Vol. 275. P. e128.
• MITOCHONDRIAL GENOME MUTATIONS ASSOCIATED WITH MYOCARDIAL INFARCTION Sazonova M.A., Ryzhkova A.I., Sobenin I.A., Orekhov A.N., Sinyov V.V., Melnichenko A.A., Galitsyna E.V., Demakova N.A., Shkurat T.P. Disease Markers. 2018. Vol. 2018. pp. 9749457.
• MITOCHONDRIAL DISEASES CAUSED BY MTDNA MUTATIONS: A MINI-REVIEW Ryzhkova A.I., Sazonova M.A., Sinyov V.V., Postnov A.Y., Orekhov A.N., Galitsyna E.V., Chicheva M.M., Melnichenko A.A., Shkurat T.P., Grechko A.V. Therapeutics and Clinical Risk Management. 2018. Vol. 14. pp. 1933-1942.
• CYBRID MODELS OF PATHOLOGICAL CELL PROCESSES IN DIFFERENT DISEASES Sazonova M.A., Sinyov V.V., Postnov A.Y., Sobenin I.A., Ryzhkova A.I., Galitsyna E.V., Melnichenko A.A., Orekhov A.N. Oxidative Medicine and Cellular Longevity. 2018. Vol. 2018. pp. 4647214.