Bolt Anton Igorevich

• ejaculate research;
• sperm preparation;
• ejaculate cryopreservatirvation.

By Embryological tecmbryological techniques:

• collection of oocyte-cumulus complexes;
• oocyte denudation;
• fertilization of oocytes by spermatozoa (IVI, ICSI, IMSI);
• identification and use of TESA material for oocyte fertilization/TESE">TEE
• manipulation of oocyte of ocyts and embryos;
• cultivation and quality assessment;
• embryo transfer to uterus;
• auxiliary hatch;
• trophectophectoderm derm biopsy.

• 2004 — graduated from Moscow Pedagogical State University, Faculty of Biology and Chemistry. He defended his diploma on the topic "Freezing of mouse embryos of preimplantation stages".

• 2004-2007 — worked as a researcher at the N. I. Vavilov Institute of General Genetics of the Russian Academy of Sciences in the Laboratory of Developmental Genetics. He studied the features of genomic imprinting in early mouse embryonic development. Several scientific papers have been published during his work. Participated in the youth conference. He participated in two grant projects dedicated to the involvement of genomic imprinting in embryonic development. During his work, he mastered the basic techniques of working with early mouse embryos, PCR, RT-PCR techniques, electrophoresis, laboratory work, and work in a vivarium.
• 2007-2010 — worked as a researcher at the Federal State Budgetary Institution State Scientific Center of Dermatovenerology and Cosmetology of the Ministry of Health of the Russian Federation in the Laboratory of Molecular Diagnostic Methods.ular Diagnostic Methods. He was engaged in PCR diagnostics of STIs. He taught a practical course on "Molecular methods of STI diagnosis" for residents.
• He worked on reagents from various manufacturing companies. During his work, he mastered various methods of DNA isolation, PCR techniques, and electrophoretic DNA detection.
• Participated in various conferences and seminars on embryological topics, including "Organization of quality control in the embryological laboratory" (Cecilia Sjoblom, 2013), and also participated as a teacher in workshops "Vitrification of oocytes and embryos" (M. Kuwayama, 2012).
• Publication Isaev D.A., Zayeva V.V., Bolt A.I., Volodina O.Y. Assisted human reproduction and diseases of genomic imprinting. Problems of reproduction. 2005, vol. 2, pp. 14-18.
• Publication Bolt A.I., Volodina O.Y. Influence of transforming growth factor alpha on the effects of genomic imprinting in mouse embryogenesis. BIOLOGY IS A SCIENCE OF THE XXI CENTURY: The 10th Pushchino School-conference of young scientists dedicated to the 50th anniversary of the Pushchino Scientific Center of the Russian Academy of Sciences (Pushchino, April 17-21, 2006). Collection of abstracts. 2006, p. 129
• Publication Bolt A.I., Volodina O.Y. Parthenogenetic mouse embryos as a model for correcting genomic imprinting pathologies. Fundamental science and clinical medicine. Tenth All-Russian Medical and Biological Conference of Young Researchers "Man and his Health" (St. Petersburg). Collection of abstracts. 2007, pp. 45-46
• He is a member of the Russian Association of Human Reproduction (RARCH).